Not for resale without express authorization. Anti-beta Actin Antibody [BA3R] (A85272) This product is identical to Thermo (MA5-15739). Long-term and scalable supply - powered by recombinant technology for fast production. . The PA5-16914 immunogen is synthetic peptide corresponding to N-terminus of human beta-actin. Test - skip launchJs Popular; Applications & Techniques . Do Beta-Actin antibodies come in lyophilized format? The antibody (also known as anti--Sm-1) is specific for the single isoform of -smooth muscle actin. (MA5-16410) beta Actin Monoclonal Antibody (SP124) Antibody info; Additional info; Supplier Invitrogen Antibodies. Conjugated Antibodies; Sample Size Antibodies . Our beta Actin monoclonal, polyclonal and recombinant monoclonal antibodies are developed in Mouse and Rabbit. Beta actin is commonly used as a western blotting loading control because it is ubiquitously expressed to high levels and is stable under most experimental conditions. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. View Rabbit Polyclonal anti-beta-Actin Antibody [DyLight 488] (NB600-505G). A specific band was detected for alpha Smooth Muscle Actin at approximately 49 kDa (as indicated) using 10 g/mL of Mouse Anti-Human/Mouse/Rat alpha Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420). Compare beta Actin Western Blot Antibody Products from leading suppliers on Biocompare. beta Actin Loading Control Antibody (BA3R), MA5-15739-1MG, from Invitrogen. Tissue culture cells -- fix with formaldehyde, treat with methanol or acetone. Beta actin is one of six actin isoforms which have been identified. Success from the first experiment - confirmed specificity . Validated Species: Human, Mouse, Rat + more. $423.50 / Each; Qty . There are six different actin isoforms in human. in a 4-20% Tris-Glycine mini-gel with the Thermo Mini gel boxes. This antibody has been shown to work in applications such as: Immunocytochemistry, Immunohistochemistry, Immunohistochemistry - fixed, Immunoprecipitation, and Western Blot. This mAb is also available as a fluorescent conjugate. Antibodies and their protocols make powerful detection reagents, which use acetyl lysine monoclonal, anti-phosphotyrosine monoclonal antibody, ubiquitin antibody, sumo antibody, anti-sumo, anti-ubiquitin, antibody, anti-cofilin, cofilin, anti-pan actin, pan actin, anti-kinesin heavy chain, Kinesin heavy chain, kinesin, drosophila Kinesin, KHC, anti-tubulin, anti-alpha/beta tubulin, anti-beta . Incubate membrane with: loading control rabbit anti beta-actin (antibodies-online, ABIN962807) diluted 1:500 in blocking buffer ON at 4C. The beta Actin Antibody was generated using Actb, and Actin, beta as the antigen. No alerts have been found for beta Actin Monoclonal Antibody (AC-15). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. 46 reviews. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Conjugated Antibodies; Sample Size Antibodies . Aliquoting is unnecessary for -20oC storage. Stable for one year after shipment. Figure Lengend Snippet: Post mortem AD tissue stained with LM229 and (A,C) and double staining with AT8 (B) and beta-amyloid antibodies (D), suggesting binding to tau rather than amyloid aggregates. Thermo Fisher mouse anti beta iii tubulin Mouse Anti Beta Iii Tubulin, supplied by Thermo Fisher, used in various techniques. When stored at 2-80C, this antibody is stable for 24 months. Discover more for less. The following were used as loading controls: rabbit anti-beta actin (ab8227) (Abcam, Cambridge, UK) 1:5000 or mouse anti-beta actin [AC-15 . This antibody has been shown to work in applications such as: EIA, Immunoassay, ELISA, Flow Cytometry, Immunocytochemistry . Primary Antibodies. COVID-19 Research Tools; Antibodies. Validated Species: Human, Mouse, Rat + more. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin . Type Antibody Clone AC-15 Conjugate Unconjugated Immunogen synthetic peptide DDDIAALVIDNGSGL conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta). Thermo Fisher Scientific's beta Actin Loading Control Antibody is a Mouse monoclonal antibody. Antibody fraction purified from rabbit anti-serum. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey, Mink, D. melanogaster, Zebrafish, Bovine Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. I've been trying to blot for beta-actin on cell culture protein lysates for weeks without success. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Beta actin is one of six isoforms of actin that have been identified in mammals. It is present in the cytoplasm. Gene Symbols: ACTB: Immunogen: KLH-conjugated linear peptide corresponding to the the N-terminal sequence of human beta-Actin. 1:500, Abcam 288 [14C8] (Cambridge, UK) 1:500. MA5-16308) at a . In humans, this protein is encoded by the gene ACTB. such as Western blotting. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog . No band was observed when beta actin knockout samples were used. beta Actin Antibody, PA5-98583, from Invitrogen. Mild membrane depolarization in neurons induces immediate early gene transcription and acutely subdues responses to successive stimulus. Cofilin 1 Proper Citation: (Thermo Fisher Scientific Cat# AM4302, RRID:AB_2536382) Target Antigen: beta Actin . Santa Cruz anti-beta-actin antibodies served as loading controls in A549 whole cell lysates, HUVEC and dermal fibroblast lysates ( sc-47778) . Alternative splicing results in multiple transcript variants. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Anti-Actin, beta antibodies are available from several suppliers. This is a Validated Antibody Database (VAD) review about human beta-actin, based on 1718 published articles (read how Labome selects the articles), using beta-actin antibody in all methods.It is aimed to help Labome visitors find the most suited beta-actin antibody. Beta actin antibodies are a specific and useful tool in studying the intracellular distribution of beta actin and the static and dynamic aspects of the cytoskeleton. ZERO BIAS - scores, article reviews, protocol conditions and more These antibodies target beta Actin in Human, Mouse, Rat, Porcine and Non-human primate samples. The membrane was probed with a beta-actin polyclonal antibody (Product # PA1-183) at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP detection reagent (Product # 21230) at a dilution of 1:250 for at least 1 hour. . Wash membrane 3x for 10min with PBS/.05%Tween-20. Validated Species: Human, Mouse, Rat + more. Due to their small size, camelid antibodies are able to detect certain epitopes that may not be accessible with a traditional antibody. Species Reactivity: Zebrafish; Applications: ELISA, Western Blot Shop beta Actin Rabbit anti-Zebrafish, Polyclonal, . Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. The Applied Biosystems Human ACTB (actin, beta) Endogenous Control (FAM Dye MGB Probe, Non-Primer Limited) is intended as an endogenous control. It reacts with Human, Bovine, Canine, Chicken/Bird, Drosophila/Arthropod, Feline, Guinea Pig, Porcine, Rabbit, and Sheep. Manufacturer: Thermo Scientific PA598583 Catalog No. Get better batch-to-batch reproducibility with a recombinant antibody. Species Reactivity: Chicken, Human, Mouse, Rabbit, Rat; Applications: ELISA, Flow . A large selection of Alpha, Beta, Gamma, Delta, Kappa, and IHU Spike variants ; Scalable to meet manufacturing needs; High purity and documented lot-to-lot consistency; . Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. 10068-1-AP targets Beta Tubulin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples. As such, primary antibodies are great tools . There are six different actin isoforms in human. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. The protein may also be known as BRWS1, PS1TP5BP1, actin, cytoplasmic 1, and I (2)-actin. The reported amino acid length is 375 and the expected mass is 41.7 kDa. It specifically labels -actin in a wide variety of tissues and species. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. These antibodies have been verified by Relative expression, Cell treatment and Knockdown to confirm specificity to beta Actin. Comprehensive assessment of estrogen receptor beta antibodies in cancer cell line models and tissue reveals critical limitations in reagent specificity . Thermo Fisher 3747 N. Meridian Road PO Box 117 Rockford, IL 61105 USA (815) 968-0747 OR (800) 874-3723 (815) 968-7316 fax Do not aliquot the antibody. Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874. Host Rabbit Type Primary Clonality Monoclonal (SP124) Conjugate Unconjugated Target Actin, cytoplasmic 1 UniProt P60709 - ACTB_HUMAN. Antibody [MA5-33078] beta Actin Recombinant Rabbit Monoclonal Antibody (RM112) by Invitrogen Antibodies. A secondary antibody is an antibody designed to target a primary antibody. The primary antibody I used was A5316 from Sigma. Search All . Smooth Muscle Actin Antibody (B4) is available as both the non . Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Storage and Stability: Store vial at 40C. COVID-19 Research Tools; Antibodies. OR Prediluted antibody which is ready-to-use for immunohistochemical staining. Thermo Fisher anti-beta-actin antibody was used as a loading control with human carcinoma cell lines to investigate the role of Brachyury in tumor cell epithelial-mesenchymal transition and tumor . View Rabbit Polyclonal anti-beta-Actin Antibody [DyLight 405] (NB600-505V). Antibodies used to identify . Many secondary antibodies are conjugated to other molecules, like Alexa Fluor dyes or . Not for use in diagnostic procedures. Form Liquid Concentration 2.6 mg/mL Storage buffer Ascites Contains 15mM sodium azide Storage conditions Store at 4C short term. The two bands you are getting might not be of same intensity and probably your beta-actin antibody is polyclonal so the 2nd band is coming because of non-specific binding of the antibody. I am trying to do western blotting for beta-actin. I used 7.5% gel,and electrophoresis as 100v for 1.5h,transfered as 15v for 1.5h by BIO-RAD semi dry.Blocking in room temperature for 1h.Primary antibody is 750: . Rabbit Polyclonal Antibody. Primary antibodies can also be raised to recognise post translational modifications such as phosphorylation, acetylation, methylation, and glycosylation. Not for use in diagnostic procedures. Mutations in this gene are associated with DFNA20/26, a subtype of autosomal dominant non-syndromic sensorineural progressive hearing loss and also with Baraitser-Winter syndrome. Gene ACTB Modification Unmodified No rating or validation information has been found for beta Actin Monoclonal Antibody (AC-15). Antibody to beta-actin provides a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton. Actin is the primary component of the cytoskeleton, and beta actin is predominantly expressed in non-muscle cells, where it controls cell structure and motility. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. Store at -20C. Variants of this antigen may also be found in other species . Quantity: 100 g: Research Discipline . Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Beta Tubulin loading control antibody (Cat. Ab6276 was shown to specifically react with beta actin in wild-type HAP1 cells. Fixed with 90% MeOH blocked with 3% BSA (30 min). For Research Use Only. . beta-Actin: Classification: Monoclonal: Formulation: Purified mouse monoclonal IgG1 antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide. View Rabbit Polyclonal anti-beta-Actin Antibody [DyLight 405] (NB600-532V). Lane 2: Beta actin knockout HAP1 cell lysate (20 g) Lanes 1 and 2: Merged signal (red and green). beta Actin Antibody Product Data Sheet Product Specific Information General Information PA1-21167 detects beta-actin in human, mouse, rat, bovine, sheep, rabbit, . Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. It reacts specifically with -smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Menu Sign in or Register Search; Custom Suppliers; Data; Citations; Images; Listing; Contact; Sign in or register to save this reagent to your favourites Search (MA5-33078) beta Actin . Ig (beta actin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. What is the theoretical molecular weight for Beta-Actin antibodies? with a primary antibody . Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. All lanes : Alexa Fluor 790 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab184576) at 1 g/ml Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole . ACT-5; ACTA1; actb; actb.L; actb1; actba; actin; actin beta; actin, alpha 1, skeletal muscle; actin, beta; actin, beta 1; actin, beta L homeolog; actin . Primary Antibodies. Due to the high sequence identity between the actin isoforms, -Actin Antibody may cross-react with other actin isoforms. Green - beta actin, ab6276 observed at 42 kDa. Do Beta-Actin antibodies come in lyophilized format? . Synonyms. ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form. Quality Invitrogen beta actin antibodies are available for a variety of research needs. Actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms. Thermo Fisher Scientific's beta Actin Antibody is a Rabbit Polyclonal antibody. L26 Thermo Fisher tonsil Yes Citrate EDTA CD21 CD 21, C3DR, CR2 EP3093 Abcam tonsil Yes Citrate EDTA CD31 PECAM-1 EP3095 Abcam tonsil Yes Citrate EDTA CD44 Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24 IM7 BioLegend tonsil Yes Citrate CD45RO - UCHL1 BioLegend tonsil Yes Citrate EDTA CD68 GP110, LAMP4, SCARD1 KP1 Thermo Fisher tonsil Yes Citrate EDTA For Research Use Only. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. This gene is located on human chromosome 7p22.1. This gene encodes one of six different actin proteins. Thermo Fisher Scientific's beta Actin Antibody is a Mouse monoclonal antibody. PIPA598583. It allows relative gene expression quantification in cDNA samples when used with other TaqMan Gene Expression Assays. Images were taken at 60X magnification. Smooth Muscle Actin Antibody (B4) is a high quality monoclonal Smooth Muscle Actin antibody (also designated Alpha Smooth Muscle Actin antibody) suitable for the detection of the Smooth Muscle Actin protein of mouse, rat, human and chicken origin. . https://www.bioz.com/result/hrp conjugated mouse anti beta actin monoclonal antibody/product/Thermo Fisher Average 86 stars, based on 1 article reviews Price from $9.99 to $1999.99 hrp conjugated mouse anti beta actin monoclonal antibody - by Bioz Stars , 2022-08 86 / 100 stars Buy from Supplier Actin gamma 1, encoded by this gene, is a cytoplasmic actin found in all cell types. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Data and Source Information . 100% Guarantee Price Match Guarantee 10g 100g 200g Quantity: Actins are highly conserved proteins that are involved in cell motility, structure and integrity. 1X10^6 HepG2 cells were stained with 0.2ug beta actin antibody (60008-1-Ig, red) and control antibody (blue). Anti-beta Actin antibody [SP124] (ab115777) Research with confidence - consistent and reproducible results with every batch. Catalog # PA1-46296 beta Actin Polyclonal Antibody Product Details Size 50 L Species Reactivity Avian, Fish, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat Host / Isotype Rabbit / IgG Class Polyclonal Type Antibody Conjugate Unconjugated Immunogen Synthetic peptide derived from the N-terminus of Human beta-Actin. Flow cytometry analysis of Beta Tubulin in CEM cells (green) compared to an isotype control (blue). No. It reacts with Human, Mouse, Rat, Chicken/Bird, and Rabbit. We offer: Secondary antibodies are often used in combination with primary antibodies to detect target proteins in various immunoassays, like western blots, ELISA, and immunofluorescence. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. Search Thermo Fisher Scientific. There are six different actin isoforms in human. Cells were probed without (control) or with or an antibody recognizing Actin (Product # MA1-744) at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). What is the theoretical molecular weight for Beta-Actin antibodies? Validated Applications: WB, IHC, ICC/IF (-). Validated Applications: WB, ELISA, ICC/IF, IHC. Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874. primary rabbit anti-MR1 antibody (antibodies-online, ABIN1537116, lot SA111213CH) diluted 1:1000 in blocking buffer ON at 4C. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. This is the the WB pic of -Actin. View specifications, prices, citations, reviews, and more. Beta actin is one of six actin isoforms which have been identified. Beta actins are cytoplasmic proteins ubuquitously . ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. However, beta actin may not be suitable for use in samples from tissues containing high actin levels, like skeletal muscle, due to potential oversaturation of signal. Validated Applications: WB, ELISA, ICC/IF, IHC. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080). The beta Actin Loading Control Antibody was generated using Actb, and Actin, beta as the antigen. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Reliably and specifically detect actin using this Anti-Actin Antibody, clone C4. Details Cells were harvested, adjusted to a concentration of 1-5x10 6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Overview Specifications Images Highlighted for "beta Actin Antibody" 80 View Datasheet Mouse monoclonal (BA3R) antibody to beta Actin for Dot, ELISA, IS and WB. Red - loading control, ab181602 observed at 37 kDa. Bioz Stars score: 96/100, based on 1 PubMed citations. It is a cytoskeletal housekeeping protein. Beta actins are cytoplasmic proteins ubuquitously . Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Primary antibodies can be raised against any antigen of research interest, including: proteins, peptides, carbohydrates, and other small molecules. Recognizes an epitope located on the N-terminal end of the -isoform of actin. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Purified anti--actin [2F1-1]; Isotype: Mouse IgG2b, ; Reactivity: Human, Mouse, Rat; Apps: WB, IF; Size: 100 g<B>IgGy Antibody Selector</B> - Quickly search hundreds of thousands of antibodies available for purchase from VWR by selecting common antibody features like antigen symbol and name, reactivity, clonality, conjugation, host, and other key factors. Prepared in 10mM PBS, pH 7.6, with 0.2% BSA and 15mM sodium azide. In The Journal of Biological Chemistry on 18 July 2022 by Rienecker, K. D. A., Poston, R. G., et al.. Article Snippet: Mouse monoclonal AT8 (MN1020) or AT100 (MN1060) were from ThermoFisher and used at 1:1000. Probe is labeled with 6FAM dye - MGB and the primers are not limited. This highly published monoclonal antibody is validated for use in ELISA, IC, IF, IH, IH (P) & WB. Monoclonal Anti--Actin (mouse IgG1 isotype) is derived from the AC-15 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. I used 1:5000 dilution first and then 1:1000 dilution (5% BSA in 1X TBST was used as diluent . . Protocol Specificity / Sensitivity -Actin Antibody detects endogenous levels of -actin. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. ACTB (actin ) gene codes for -actin. (MA5-15739-D680) beta Actin Loading Control Monoclonal Antibody (BA3R), DyLight 680 - Invitrogen Antibodies - CiteAb Actins are highly conserved proteins that are involved in cell motility, structure and integrity.

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